Analysis Note

ControlRaji cell lysate

Application

Research CategoryApoptosis & Cancer

Research Sub CategoryBCL2 & Inhibition

Detect Mcl-1 using this Anti-Mcl-1 Antibody, clone RC13 validated for use in WB, IH & IP.

Immunohistochemistry Analysis: A previous lot was used by an independent laboratory in follicular lymphoma, diffuse large cell lymphoma, and polymorphic lymphoma tissues. (Zhou, P., et al. (2001). Blood. 97(12):3902-3909).

Immunoprecipitation Analysis: A previous lot was used by an independent laboratory in IP (Han, J., et al. (2005). Biological Chemistry. 280:16383-16392).

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

Mcl-1 (Induced myeloid leukemia cell differentiation protein) is a potential transmembrane protein associated with the mitochondria belonging to the Bcl-2 family of proteins. Members of the Bcl-2 family have known participation in regulatory roles for apoptosis, cell growth, and differentiation. The hydrophobic carboxyl terminus is conserved among members of this family and in other members has proven to be a transmembrane region. Mcl-1 is found to be expressed early in myeloid differentiation. Two separate isoforms of Mcl-1 exist with opposing functions. Full length Mcl-1 (Isoform 1) promotes cell viability, over proliferation. Isoform 2 results from the skipping of the central exon of Mcl-1 during mRNA processing and the joining of the first and third exons. The function of the resulting protein induces apoptosis when accumulated. Pro-apoptotic function is shown to increase when isoform 1 and 2 dimerize.

Immunogen

Recombinant protein corresponding to human Mcl-1.

Linkage

Replaces: MAB4602

Physical form

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Protein G Purified

Format: Purified

Quality

Evaluated by Western Blot in Raji cell lysate.

Western Blot Analysis: A 1:1,000 dilution of this antibody detected Mcl-1 in 10 µg of Raji cell lysate.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Target description

~37 kDa observed